Cusabio Organism Recombinants

Abstract

The discoveries of the double-stranded structure of DNA and the development of recombinant DNA technology sparked a revolution in industrial microbiology. Traditional industrial microbiology merged with molecular biology to produce improved organism recombinant processes for the industrial production of primary and secondary metabolites, biopharmaceutical proteins, and industrial enzymes. New genetic techniques such as metabolic engineering, combinatorial biosynthesis and molecular breeding techniques and their modifications are greatly contributing to the development of improved industrial processes.

Furthermore, functional genomics, proteomics and metabolomics are being exploited for the discovery of new small molecules valuable for medicine, as well as enzymes for catalysis. Sequencing of industrial microbial genomes is ongoing, which bodes well for future process improvement and the discovery of new industrial products.

Keywords:

antibiotics, antitumor agents, biopharmaceuticals, hypocholesterolemic agents, enzymes, genetic recombination, industrial products, primary metabolites, secondary metabolites

Amino acids.

Genetic engineering has impacted amino acid production through the use of the following strategies: (1) amplification of a rate-limiting enzyme pathway; (2) amplification of the first enzyme after a branch point; (3) cloning of a gene encoding an enzyme with more or less feedback regulation; (4) introduction of a gene encoding an enzyme with a functional or energetic advantage as a replacement for the normal enzyme; and (5) amplification of the first driving enzyme from central metabolism to increase carbon flux in the pathway followed by sequential removal of bottlenecks caused by the accumulation of intermediates.

Transport mutations have also become useful, that is, a mutation that reduces amino acid uptake allows for enhanced excretion and lower intracellular feedback control. This has been especially useful in the production of tryptophan and threonine. In cases where excretion is mediated by transporters, increased activity of these transporter enzymes increases amino acid production.

Other compounds.

Nucleosides. Genetic engineering of the inosine monophosphate (IMP) dehydrogenase gene in a B. subtilis strain that produces 7 gl−1 desirable guanosine and 19 gl−1 undesirable inosines changed the production to 20 gl−1 guanosine and 5 gl −1 inosine. Guanosine is used to produce guanosine monophosphate (GMP), a powerful flavouring agent.

Carotenoids. Carotenoids were overproduced by the introduction of Erwinia uredovora carotenoid gene clusters into E. coli and overexpression of E. coli deoxyglucose phosphate synthase, the key enzyme of the non-mevalonate isoprenoid biosynthetic pathway zeaxanthin at 0.6 mg g−1.

Glucosamine. Glucosamine is used for osteoarthritis and is produced by acid hydrolysis of chitin from shellfish waste. Since many patients have shellfish allergies, a microbial source is desirable. Metabolic engineering of E. coli produced a process that produced 17 gl−1 of glucosamine. In this strain, glucosamine synthase was overexpressed, glucosamine-degrading genes were inactivated, and glucosamine inhibition of glucosamine synthase was decreased. by mutational modification of the enzyme through error-prone PCR. Overexpression of a heterologous glucosamine-6-P-N acetyltransferase produced a strain that produces 110 g l -1 of N-acetylglucosamine which is readily converted to glucosamine by mild acid hydrolysis.

Solvents. Cloning it’s as (acetone) ADC operon genes (encoding acetoacetate decarboxylase), and CTF and CTF (two genes encoding coenzyme A transferase) into a plasmid containing the ADC promoter in Clostridium acetobutylicum resulted in a 95% increase % in the production. The increases were 95% in acetone production, 37% in butanol, 90% in ethanol, 50% yield of solvent from glucose, and 22-fold lower production of undesirable acids. The introduction of the acetone operon of C acetobutylicum in E. coli led to the high production of acetone by the latter.

Cusabio Signal Transduction Recombinants

Product details

Purity: >85% (SDS-PAGE)

Destination names: STAM

Product name: Signal Transduction Recombinants

Uniprot No.: Q92783

Alternative Names

DKFZp686J2352; HSE1 homologue; OTTHUMP00000019237; signal transducer adapter molecule 1; signal transducing adapter molecule (SH3 domain and ITAM motif) 1; STAMS 1; STAM; STAM-1; STAM1_HUMAN

Species: Homo sapiens (Human)

Expression Region: 2-540

Protein length: Total length of the mature protein

Label information

The following labels are available.

  • N-terminus His-tagged
  • Without tags
  • The type of label will be determined during the production process. If you have specified a tag type, let us know and we will develop the specified tag preferentially.

Form: Lyophilized powder

Buffer before lyophilization: Tris/PBS based buffer, 6% trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged before opening to bring the contents to the bottom. Reconstitute protein in sterile deionized water at a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and an aliquot for long-term storage at -20℃/-80℃. Our final default glycerol concentration is 50%. Customers could use it for reference.


Storage Conditions

Store at -20°C/-80°C upon receipt, need to be aliquoted for multiple uses. Avoid repeated cycles of freezing and thawing.

Shelf life

Shelf life is related to many factors, storage condition, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.

Delivery time

The delivery time may differ depending on the way or location of purchase, consult your local distributors for the specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Cusabio Research Recombinants

Description

For more than 14 years, the CUSABIO Protein Expression Platform has established five Research recombinant expression systems from prokaryotes (E.coli) to eukaryotes (insect baculovirus, mammalian cells and yeast), and has also created a protein expression system Unique in vitro E.coli, which allows expressing transmembrane proteins that are normally quite difficult to express. In addition, the CUSABIO protein quality control department owns a professional technical team, equipped with advanced experimental apparatus, to ensure that each protein has a complete and high-quality COA report. CUSABIO purified proteins exceed 90% purity as detected by SDS-PAGE analysis.

We currently have 67 native proteins, 100 small molecule antigens, 640 active proteins, more than 4,400 recombinant proteins in stock, more than 8,900 recombinant proteins developed, more than 13,000 cDNA clones, more than 36,000 transmembrane proteins, more than 500,000 semi-custom recombinant proteins and we could also provide risk-free protein expression service and gene and oligo synthesis service. All these products belong to our Line of Protein Products.

Why choose us?

1. 100% risk-free performance guaranteed

CUSABIO protein products are carefully validated under strict quality control standards to ensure performance. If the product doesn’t work as promised in the manual, just contact us before the expiration date for technical support, replacement or refund.

2. Strict quality control process

The quality of our proteins is the cornerstone of our ability to serve our customers. For this reason, we invest enormous resources in the development of proteins, validating their application, managing, controlling and improving quality. Each protein will go through our standardized quality control process before shipping.

  • Protein concentration detection
  • molecular detection
  • purity detection
  • Endotoxin detection
  • Detection of biological activity (This procedure only applies to proteins with validated activity)
  • Sequence Accuracy

3. Customer Testimonials

I hope this email finds you well. I recently ordered 50ug of Human Protein Carbonic anhydrase 9 (CA9) and I am very pleased with it. We have ordered this protein 5 times in total and all batches perform well. Now we would like to order 10mg and we think the new lot will also do well in our detection.

4. Characteristics of the five expression systems

We have five recombinant expression systems, including yeast, E. coli, baculovirus-infected insect cell, mammalian cell, and cell-free (E. coli in vitro). Different expression systems have various characteristics and are suitable for expressing different proteins, which can satisfy different experimental requirements of researchers. Here is an introduction to the characteristics of five expression systems that might help you choose a suitable system.

5. Multiple Labels for Recombinant Protein

We could provide multiple labels for each protein to meet the different needs of different customers. Here we list the possible tag types for each expression system.

6. Bulk order

  • Capacity

Large fermentation tanks (50L, 500L) allow us to produce proteins on a large scale. We have successfully completed industrial bulk orders for 100mg, 200mg and 500mg.

  • Order application

You can use the protein name/catalogue number/alias/abbreviations/Uniprot number to search for your desired protein in the above search bar, which contains millions of proteins, then you can request the quotation via live chat or send your request to protein1@cusabio. com. If you can’t search for the protein of your interest, please fill in the “Custom Protein Request Form” online, your information will be strictly protected and will not be disclosed to third parties, we will reply to you by email within 24 hours.

7. Professional R&D team

Our company has an R&D team made up of scientists with doctoral and master’s degrees. More than 60% of the total number of employees are in the technical service team, including 20% ​​with PhD degrees and 40% with Master’s degrees; The technical elites are from recognized national institutions, with years of experience in protein development.

8. Animal free

CUSABIO is pleased to inform you that we have developed our line of animal-free recombinant proteins, which drastically minimize variable factors and the possible contamination of mammalian pathogens during the production process. These animal-free proteins are produced in animal-free media, and the purification kit is also free of animal components. Animal-free recombinant protein is worth owning!

9. Good Manufacturing Practice (GMP) Grade Proteins

  • 4000㎡ research laboratories
  • 2000㎡ GMP level production workshop

10. The number of new products

Our protein expression platform has established five prokaryotic to eukaryotic recombinant expression systems and has also built a cell-free expression system. We also established a special and independent protein quality control department, which meets the quality standard of the international protein market, to detect purity, activity, endotoxin level, etc. of the proteins. With our professional R&D team, we can develop and produce more than 2700 proteins every year.

11. Multiple delivery forms

  • Liquid form

The default delivery form is liquid form. If the customer has demand for freeze-dried form, please note this requirement when placing the order.

  • Lyophilized powder

Relatively speaking, the lyophilized form will be more stable than the liquid form and the shelf life will be longer. But freeze-drying can damage the protein structure and can affect the activity of some proteins.

Additional services

1. Label removal service

  • If you need to remove the tag, please contact us in advance; otherwise, we will not remove the tag.
  • Not all protein tags can be removed, as some proteins will be highly unstable after tag removal.
  • If we manage to remove the label, we will charge an additional cost.
  • If we are unable to remove the label, we will not charge any additional costs.
  • Generally, the delivery time will be extended by 2-3 days.

2. Western Transfer Service

  • We could offer Western Blot service to label antibodies for free. If the customer has a demand, please provide this information when placing the order.
  • Generally, the delivery time will be extended by 2-3 days.
  • The WB detection result will be displayed in COA.